Coding

Part:BBa_K1293022:Design

Designed by: Sandor Ban   Group: iGEM14_HUNGENIOUS   (2014-06-27)


Pure chitinase gene (Cv2935) of Chromobacterium violaceum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 232
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 1149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The synthesis of this gene still unsolved.


Source

The part's sequence is coming from genomic DNA of Chromobacterium violaceum.

References

Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli (Lobo et al; BMC Biotechnology 2013, 13:46 doi:10.1186/1472-6750-13-46)